Monoclonal antibody to dengue capsid protein

Abstract

Dengue Fever (DF) and Dengue Hemorrhagic Fever/Dengue Shock Syndrome (DHF/DSS) are considered the most important arthropod-borne viral diseases in terms of morbidity and mortality. The emergency and severity of dengue (Den) infections increase the necessity of an early, quick and effective dengue laboratory diagnostic. Viral isolation is considered a gold standard for diagnosis dengue infection using monoclonal antibodies (mAbs) as a tool for determining serotype specificity. Alternatives have been used to improve sensitivity and time in dengue diagnosis. Based on the early expression of dengue C protein in the life cycle, we focused our study in the application of an anti-dengue 2 virus capsid protein mab in dengue diagnosis. The kinetic expression of dengue-2 capsid in mosquito cells and its immuno-localization in experimentally infected suckling albin Swiss (OF-1) mice brain tissues was established. The results demonstrate the utility of this mAb in significant early dengue diagnosis in traditional isolation. On the other hand, a preliminary study of an enzyme immunoassay method using 8H8 mab for specific detection of dengue C protein antigen was performed making possible recombinant C protein quantification. The results suggest that detection of dengue capsid protein could be useful in the diagnosis of early dengue infection.