Abstract
Monoclonal antibodies were raised to a crude photoreceptor-membrane suspension from chicken retinas. Clones producing antibodies against cone outer segments were selected by screening with immunocytochemistry on semithin sections of the retina. One monoclonal antibody, called COS-1, specifically labelled outer segments of double cones and one type of single cones; outer segments of rods and several single cones were not stained. On immunoblots of retinal photoreceptor membranes, this antibody recognized a protein with an apparent molecular mass of 33000. The visual pigment character of the 33000 protein was indirectly established by another monoclonal antibody, OS-2, which labelled all outer segments on semithin sections and four bands (33000-, 36000-, 38000–40000- and a composite band between 66000–72000 MW) on immunoblots. Of these, the 36000- and the 72000 MW protein bands were identified with an anti-rhodopsin polyclonal antibody as rhodopsin monomer and dimer. Monoclonal antibody OS-2 is assumed therefore to represent an antibody against a common epitope of all visual pigments of the chicken. The monoclonal antibody COS-1 was found to bind to certain cone outer segments of many other vertebrate species as well.
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