Abstract
Dengue virus (DENV) is the world most prevalent mosquito-transmitted virus. The incidence of dengue infection has been increasing and most of the infected people are asymptomatic or have non specific febrile illness. Laboratory test is essential to confirm dengue infection in epidemiological studies. We developed an indirect ELISA test based on monoclonal immunoglobulin against all four DENV serotypes and evaluated the test for the diagnosis of asymptomatic dengue infection in paired annual serum samples. The indirect ELISA was found to have a sensitivity 87.5% and specificity 78.3% at optimized cut off. The results from indirect ELISA demonstrate an incidence of asymptomatic dengue infection from children aged 4 - 11 years in Ratchaburi province, Thailand (2006-2009). These findings indicate that the indirect ELISA is suitable for serodiagnosis and seroepidemiological studies of asymptomatic dengue infection.
Highlights
Dengue virus (DENV) is a major arbovirus responsible for infection in an estimated 2.5 - 3.0 billion people at risk worldwide
We developed an indirect enzyme-linked immunosorbent assay (ELISA) test based on monoclonal immunoglobulin against all four serotypes of DENV to diagnosis asymptomatic dengue infection in paired annual serum samples in a dengue endemic area in Thailand comparing to plaque reduction neutralization test (PRNT)
To evaluate the cut off criterion for PRNT50, 22 paired annual serum samples of RT-PCR proven symptomatic dengue infection cases and 72 paired annual serum samples of children with no history of symptomatic infection were tested by PRNT50
Summary
Dengue virus (DENV) is a major arbovirus responsible for infection in an estimated 2.5 - 3.0 billion people at risk worldwide. Dengue infection may result in asymptomatic infection, dengue fever (DF), dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS) [1]-[4]. The pathogenesis of disease severity is not completely understood [5]-[7]. Infection with one serotype can cause life-long immunity. (2016) Monoclonal Antibody-Based Enzyme Immunoassay for Epidemiological Studies of Asymptomatic Dengue Infection. Asymptomatic infection is the most common clinical syndrome of dengue and may have a role in dengue transmission [8]-[12]. Epidemiological studies on asymptomatic dengue infection are essential and the incidence of asymptomatic dengue infection reflects the quality of dengue control. Patterns of pre-existing immunity between asymptomatic and symptomatic dengue infection may provide insight on the pathogenesis of dengue disease
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