Monoclonal Antibody Against Stromal Cell-Derived Factor 2.

Abstract

Monoclonal Antibodies in Immunodiagnosis and ImmunotherapyVol. 39, No. 1 Monoclonal AntibodiesOpen AccessCreative Commons licenseMonoclonal Antibody Against Stromal Cell-Derived Factor 2Published Online:14 Feb 2020https://doi.org/10.1089/mab.2019.0046AboutSectionsPDF/EPUB Permissions & CitationsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail Antigen Used for ImmunizationA synthetic peptide of 20 amino acids (GIFMKPSELLKAEAHHAELC), which corresponded to residues 193–211 of human stromal cell-derived factor 2 (SDF-2).Method of ImmunizationThe rat lymph node method established by Sado et al.(2)Parental Cell Line Used for FusionMouse myeloma SP2 cells.Selection and Cloning ProcedureThe hybridoma supernatants were screened using an enzyme linked immunosorbent assay (ELISA) against the human SDF-2 synthetic peptide. Positive clones were subcloned and rescreened by ELISA, immunoblotting, and immunoprecipitation.Heavy and Light Chains of ImmunoglobulinRat IgG2ακ.SpecificityWe determined specificity of MAb by immunoblotting analysis using the microsomes of SDF-2-knockout OCUM-2M/OXA cells. Our MAb gave specific signals as a 23 kDa band in the wild-type cells only.Specific Antigen IdentifiedWe did not identify specific antigen. However, it would include 20 amino acids (GIFMKPSELLKAEAHHAELC), which corresponded to residues 193–211 of human SDF-2.Availability YesNoTissue culture supernatant[ ][✓]Ascitic fluid[ ][✓]Hybridoma cells[✓][ ]