Monoclonal antibodies to the epitope α-Gal-(1-4)-β-Gal-(1- ofMoraxella catarrhalisLPS react with a similar epitope in type IV pili ofNeisseria meningitidis

Abstract

Murine monoclonal antibodies (MAbs) against the A, B and C LPS serotypes ofM. catarrhaliswere generated and their binding specificity was examined in an enzyme-linked immunosorbent assay (ELISA). Two broadly cross-reactive monoclonal antibodies (MCA1 and MCC2) against the outer core region of LPS were further characterized. A panel of synthetic glycoproteins and glycolipids was used to determine the binding specificity of the MAbs. MCA1 and MCC2 bound specifically to α-Gal-(1-4)-β-Gal of galabiose and globotriose glycoconjugates. The reactivity of the MAbs with galabiose was higher than that with globotriose. The MAbs could recognize the α-Gal-(1-4)-β-Gal epitope only when it was in a terminal position. MCA1 was further shown to react with a similar epitope in the glycosylated type IV pili ofN. meningitidis, which has been shown to contain a 1-4 linked digalactose at the terminal part of the saccharide present in the pili. MCA1 could efficiently recognize this epitope indicating that it was exposed on the surface of the pili.