Monoclonal antibodies to human erythrocyte glucose 6-phosphate dehydrogenase

Abstract

glucose 6-phosphate dehydrogenase (G6PD, type B) have been raised in rabbits and used for the characterization of genetic variants of this enzyme protein [l-4]. Use of the antiG6PD antisera for estimating the specific activity of GGPD variants has recently been criticized and shown to be inadequate to this purpose [5]. More- over, both the specificity and strength of conventional antisera are not satisfactory enough to allow a precise characterization of G6PD variants associated with severe deficiency of activity [6]. In an attempt to overcome these limitations, we used cell fusion techniques to obtain anti-G6PD anti- body-secreting hybridomas [7]. By this approach, a number of antibodies directed to human G6PD were isolated after fusion of an 8-azaguanine-resistant mouse myeloma line with splenocytes from a mouse hyperimmunized with G6PD. 2. Materials and methods