Abstract
1. The ability of four domain-specific anti-chicken ovotransferrin antibodies to bind to turkey, quail, duck, pheasant and goose ovotransferrins was examined directly by an enzyme-linked immunoassay and indirectly in a competitive radioimmunoassay. 2. The ability of these same ovotransferrins to compete with radioiodinated chicken ovotransferrin for binding to transferrin receptors on chick embryo red blood cells was also tested. 3. The hypothesis that evolutionarily conserved determinants involved in receptor recognition can be predicted by monoclonal antibodies that block binding of ovotransferrin to receptor was found to be incorrect.
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