Monoclonal antibodies specific for Kappa chain, Lambda chain, and IgG1 of human gammaglobulin

Abstract

Hybrid cell lines secreting antibodies specific for human gammaglobulin (HGG) were prepared by cell fusion and cloning techniques. The monoclonal antibodies were tested for their ability to bind HGG fragments, purified myeloma proteins, and Bence Jones proteins. Each antibody reacts with a different antigenic determinant of HGG. One reacts with isolated kappa (κ) light chains, one with isolated lambda (λ) light chains, and one with the Fc fragment of IgG1 molecules. The reactivity patterns of two additional monoclonal antibodies are more complex. One reacts with a determinant present on the Fc of all IgG subclasses and the other binds to a determinant on the Fab of IgG molecules. The two monoclonal antibodies reactive with light chains also bind to surface components of human B cells. The murine immunoglobulin (Ig) class of each clone product was identified.