Abstract
MAb against the antigen CD117 - stem marker of human tumor cells. Strain 406 PPI prepared by cell fusion of mouse myeloma NS-1 cells with spleen mice BALB/ C, pre-immunized three times at an interval of two weeks, the cells of the cell line of human melanoma melKor. Merging conducted using a solution of PEG/DMSO. For screening received mAb 406 used human melanoma cell lines which differed in the expression of CD117 antigen FSBSI "N.N. Blokhin RCRC" collection. N.N Blokhin. Antigen expression was studied in immunofluorescence and evaluated on a flow cytometer BD FACS CantoTMII. ICA IC0-406 was compared with commercial ICA against antigen CD 117 (Germany). The results indicated the identity of the frequency of antigen-positive cases and the percentage of antigen cells. ICA IC0-406 block binding to cells melKor ICA anti-CD117. Linking ICA IC0-406 antigen-positive cells causes modulation of antigen CD 117.
Highlights
Среди опухолевых клеток имеется небольшая популяция клеток, способных к формированию дифференцированных опухолевых клеток, а также способных к собственному воспроизведению, т.е. имеющих характеристики стволовой клетки [7; 20; 22]
The results indicated the identity of the frequency of antigen-positive cases and the percentage of antigen cells
Turkina A.G., Zabotina T.N., Kusnetzov S.V. et al Studies of some mechanisms of drug resistence in chronic myeloid leukemia (CML) // Advances in Experimental Medicine and Biology. – 1999. – 457. – P. 477–88
Summary
МОНОКЛОНАЛЬНЫЕ АНТИТЕЛА ICO-406 ПРОТИВ АНТИГЕНА CD117 ФГБНУ «РОНЦ им. Получены МКА против антигена CD117 – маркера стволовых опухолевых клеток человека. Для скрининга полученных МКА ICO-406 использовались клеточные линии меланомы человека, различающиеся экспрессией антигена CD117 из коллекции ФГБНУ «РОНЦ им. МКА ICO-406 сравнивали с коммерческими МКА против антигена СD 117 (Германия). Связывание МКА ICO-406 с антиген-положительными клетками вызывает модуляцию антигена CD117. MONOCLONAL ANTIBODIES ICO-406 AGAINST THE ANTIGEN CD117 FSBSI «N.N. Blokhin Russian Cancer Research Center», Moscow. Abstract mAb against the antigen CD117 – stem marker of human tumor cells. For screening received mAb 406 used human melanoma cell lines which differed in the expression of CD117 antigen FSBSI "N.N. Blokhin RCRC" collection. Linking ICA ICO-406 antigen-positive cells causes modulation of antigen CD117. Эти клетки получили название опухолевых стволовых клеток (СОК) [14].
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