Abstract
We have established a set of mouse hybridoma clones—using the technique of Köhler and Milstein (1975)—each producing an antibody that reacts preferentially with certain transformed cell types as opposed to their normal control cells. These clones were produced in the course of experiments designed to isolate hybridoma clones that produce antibodies to feline oncornavirus-associated cell-membrane antigen (FOCMA). FOCMA is defined by indirect immunofluorescent-antibody (IFA) assays on live FL74 cells, using FOCMA typing serum (Hardy et al. 1978); it is found either on the feline sarcoma virus (FeSV)-transformed fibroblasts (Essex et al. 1971) or in lymphomas produced presumably as a result of feline leukemia virus (FeLV) infection (Essex 1976; Hardy et al. 1978).
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