Abstract
Two simple solid-phase sandwich immunoassays for human chorionic gonadotropin (hCG) employing monoclonal antibodies have been described. One is a sandwich erythro-immunoassay employing V-shaped well microtitration plates coated with monoclonal anti-beta-hCG antibody and monoclonal anti-alpha-hCG antibody labelled sheep erythrocytes. The second is a 'dot' enzyme immuno-assay employing dip-stick (plastic strips pasted with nitrocellulose pads) coated with monoclonal anti-beta-hCG antibody. Anti-alpha-hCG monoclonal-alkaline phosphatase conjugate was used to reveal hCG bound to solid surface. The assays can be performed by 'one-step' or 'two-step' procedures. Erythro-immunoassay as well as 'dot' enzyme immunoassay was able to detect in urine as low as 10 mIU hCG/ml. A good correlation was observed between the values obtained by these two methods as well as 'two-step' sandwich enzyme immunoassay on 47 urine samples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Scandinavian journal of clinical and laboratory investigation
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
