Abstract
Four mouse monoclonal antibodies generated against Brucella melitensis 16M, and three generated against B. suis 1330 were analysed. An ELISA (enzyme-linked-immunosorbent assay) with whole cells as antigens was used to determine cross-reactivities with other Gram-negative bacteria. Two antibodies showed cross-reactivity with smooth Brucella strains only. Five antibodies also reacted with Yersinia enterocolitica O:9, but not with other bacteria. Two of these antibodies had significantly higher titres with A > M serotype Brucella strains, indicating that these epitopes are related to the antigenic A complex. The antigenic determinants recognized by the monoclonal antibodies showed varying degrees of susceptibility towards oxidation. They were shown by immunoblotting to be located on the polysaccharide moiety of the O-side chain.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
