Monoclonal antibodies against the bacterial-like organism associated with citrus greening disease

Abstract

Hybridoma clones secreting specific monoclonal antibodies (mAb) against the bacterium-like organism associated with citrus greening disease were produced from homogenates of infected pholem tissues used as immunogens in mice immunizations. Differential ELISA and immunofluorescence were used to screen for hybridomas secreting mAb against the greening organism (GO). Two such hybridoma clones were obtained. The mAb were specific for the GO. No cross-reactions were seen with any of the other phloem-limited prokaryotes tested nor with healthy plant material.