Abstract
Mitochondrial respiratory chain and NADPH oxidase are described as major sources of reactive oxygen species (ROS) in diabetic cardiomyopathy. Oddly, the role of monoamine oxidase (MAO) has been overlooked so far. These mitochondrial flavoenzymes are responsible for oxidative stress and cell death in failing and ischemic/reperfused hearts. Here we assessed whether MAO contribute to high glucose (HG) induced cell damage. Neonatal rat cardiomyocytes were cultured in high glucose (HG, 30 mM) for 24 or 48 hours in the absence or presence of MAO inhibitor pargyline and compared to those cultured in normal (NG, 5 mM) or high mannitol (HM, 30mM) containing media. Mitochondrial ROS production, detected by Mitotracker Red, was significantly increased in HG myocytes (+40%, p<0.001) as compared to other cells. Pargyline completely prevented this increase. Apoptosis and necrosis, assessed by bisbenzimide and propidium iodide staining respectively, were 1.8 and 2‐fold higher in HG (p<0.05). MAO inhibition significantly reduced cell death both after 24 and 48 hours of culture in HG. In conclusion, here we demonstrate that MAO play a major role in the oxidative stress and cell death in cardiomyocytes exposed to HG. MAO may amplify the effects of existing ROS triggers or HG may directly enhance MAO activity. Both phenomena are likely to be relevant for the onset and progression of diabetic cardiomyopathy.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.