Monitoring the fate of untreated and microwave treated canola (oilseed rape) meal protein in the rumen using SDS-PAGE

Abstract

Canola meal (CM) proteins are extensively degraded in the rumen. Various physical and chemical treatments have been used to alter the rate of ruminal degradation of CM protein, thus decreasing rumen protein degradability and increasing the content of metabolizable protein. To our knowledge, little information is available concerning the effect of microwave treatment on the type of CM proteins that leaves the rumen undegraded. The main objective of our research was to evaluate the degradation of untreated and microwave treated CM proteins by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE).