Monitoring the antioxidant effects of catechin using single-walled carbon nanotubes: Comparative analysis by near-infrared absorption and near-infrared photoluminescence

Abstract

We measured the optical responses of single-walled carbon nanotubes (SWNTs) after adding Japanese green tea or catechin. SWNTs were covered with DNA in aqueous solution, and tea or catechin solution was added to the DNA–SWNT suspension. The antioxidant effects of tea and catechin were detected as changes in the near-infrared (NIR) absorption (ABS) and NIR-photoluminescence (PL) spectra of the SWNTs. Commercial Japanese tea, diluted 100 times and containing 15μg/mL catechin, was sufficient for recovering NIR-ABS and NIR-PL spectra when the DNA–SWNT suspension was pre-treated with 0.03% hydrogen peroxide(H2O2). Similar results were obtained with 15μg/mL of pure catechin solution. SWNTs with specific chirality were sensitive to the NIR-ABS and NIR-PL changes. The (10, 5)/(8, 7) and (9, 4) SWNTs showed the highest recovery in NIR-ABS and NIR-PL, respectively. NIR-PL recovery was higher than that of NIR-ABS for (10, 5)/(8, 7) and (9, 4). Spectral changes could be monitored thoroughly at pH 8.0, contrary to pH 6.0 and 7.3. However, the most dynamic recovery of NIR-ABS and NIR-PL was observed at pH 6.0. Furthermore, time-lapse measurements revealed that recovery was faster with tea or catechin addition than H2O2-induced oxidation.