Monitoring storage induced changes in the platelet proteome employing label free quantitative mass spectrometry

Maaike Rijkers,A J Gerard Jansen,Dirk De Korte,Frank W G Leebeek,Jan Voorberg,Bart L Van Den Eshof,Pieter F Van Der Meer,Floris P J Van Alphen,Alexander B Meijer

doi:10.1038/s41598-017-11643-w

Maaike Rijkers, A J Gerard Jansen + Show 7 more

Open Access

https://doi.org/10.1038/s41598-017-11643-w

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Abstract

Shelf life of platelet concentrates is limited to 5–7 days due to loss of platelet function during storage, commonly referred to as the platelet storage lesion (PSL). To get more insight into the development of the PSL, we used label free quantitative mass spectrometry to identify changes in the platelet proteome during storage. In total 2501 proteins were accurately quantified in 3 biological replicates on at least 1 of the 7 different time-points analyzed. Significant changes in levels of 21 proteins were observed over time. Gene ontology enrichment analysis of these proteins revealed that the majority of this set was involved in platelet degranulation, secretion and regulated exocytosis. Twelve of these proteins have been shown to reside in α-granules. Upon prolonged storage (13–16 days) elevated levels of α-2-macroglobulin, glycogenin and Ig μ chain C region were identified. Taken together this study identifies novel markers for monitoring of the PSL that may potentially also be used for the detection of “young” and “old” platelets in the circulation.

Highlights

Platelet transfusion is commonly used to restore platelet count and prevent bleeding in thrombocytopenic patients and patients with platelet dysfunctionality
A principle component analysis (PCA) was performed to compare the changes in the platelet proteome induced by storage to the data corresponding to the total number of proteins identified in all samples (Fig. 1a,b)
Employing Label free quantitative (LFQ) mass spectrometry we provide a data set of 2501 accurately quantified proteins that are present in stored platelets

Summary

Introduction

Platelet transfusion is commonly used to restore platelet count and prevent bleeding in thrombocytopenic patients and patients with platelet dysfunctionality. An increase in levels of reactive oxygen species leading to oxidative stress has been reported[8]. Despite these findings, triggers for initiating the development of the PLS are still not fully elucidated. In previous studies on platelet storage, only limited numbers of proteins were identified. We used LFQ mass spectrometry to monitor changes in the platelet proteome during storage.

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