Abstract
The best of both worlds: Combining a recombinant docking domain (see picture, blue ribbon) with a chemical sensing module (green hexagons) yields a highly selective ERK sensor. This probe exclusively monitors ERK1/2 activity in unfractionated cell lysates without any off-target kinase inhibitors. Good selectivity and biophysical parameters allow high-throughput analysis of ERK1/2 activity without time-consuming enzyme purification.
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