Abstract
Monitoring protein expression levels in E. coli using a high throughput approach
Highlights
We have developed a high-throughput method to rapidly identify the protein constructs which are well expressed and find out which experimental factors influence their production
The 4th Recombinant Protein Production Meeting: a comparative view on host physiology The organisers would like to thank Novozymes Delta Ltd who generously supported the meeting. Meeting abstracts – A single PDF containing all abstracts in this supplement is available here http://www.biomedcentral.com/content/pdf/1475-2859-5-S1-info.pdf
This screening is performed by a combination of small scale fermentation in deep-well blocks, cell lysis with a 24 microtips sonicator, Ni-NTA magnetic beads purification, and an automated gel capillary electrophoresis system, which allows a high-throughput and quantitative analysis of the multiple variables in one experiment
Summary
Address: Novartis Institutes for Biomedical Research, 4000 Basel, Switzerland * Corresponding author from The 4th Recombinant Protein Production Meeting: a comparative view on host physiology Barcelona, Spain. 21–23 September 2006. Address: Novartis Institutes for Biomedical Research, 4000 Basel, Switzerland * Corresponding author from The 4th Recombinant Protein Production Meeting: a comparative view on host physiology Barcelona, Spain. Published: 10 October 2006 Microbial Cell Factories 2006, 5(Suppl 1):P78 doi:10.1186/1475-2859-5-S1-P78. The 4th Recombinant Protein Production Meeting: a comparative view on host physiology The organisers would like to thank Novozymes Delta Ltd who generously supported the meeting. Meeting abstracts – A single PDF containing all abstracts in this supplement is available here http://www.biomedcentral.com/content/pdf/1475-2859-5-S1-info.pdf
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