Abstract
The purpose of the study: to identify significant changes in the electrochemical properties of quarantine-stored blood plasma and of donor blood plasma during its in vitro mixing with quarantined plasma in order to assess the effect of transfusion of quarantine-stored plasma on recipient plasma in a model experiment. Materials and methods. Blood plasma of 20 clinically healthy volunteer donors was quarantined for 6 months at -40°C. Monitoring of the redox potential (RP) of the quarantined plasma was carried out directly on the day of sampling without freezing the sample, and then on days 1, 3, 7 and 14, as well as after 1, 1.5, 2, 3, 4, 5 and 6 months of storage of frozen samples. Each of 15 blood plasma samples donated by clinically healthy volunteers was mixed in vitro with blood plasma quarantined for 6 months at a ratio 1:1, and the RP of the mixture was measured by a platinum microelectrode technique. Results. It was found that during the storage of quarantined samples at a temperature of -40°C, the blood RP shifted to more positive values in 70% of cases. Addition of the quarantined plasma to the plasma of practically healthy volunteer donors also led to a shift in the final mixture RP to positive values in 13 of 15 cases (87%). Conclusion. Significant changes in RP values have been found when measuring the RP of the quarantined blood plasma, demonstrating predominantly oxidative processes in the plasma. Since significant shift of RP in blood plasma to the positive values has been associated with the deterioration of the patient's state, we concluded that further clinical studies on the use of quarantined plasma with high positive RP values are warranted.
Highlights
At present, plasma transfusion is widely used in clinical practice to treat patients with massive blood loss
The stored samples were examined on day 1, 3, 7, 14 and after 1, 1.5, 2, 3, 4, 5, and 6 months; for this purpose, the test tube with a sample was thawed according to the conditions described in [3], and the redox potential (RP) of the thawed blood plasma sample was measured at a temperature of 25°C
Results of blood plasma RP monitoring during quarantine are presented on fig. 1
Summary
Plasma transfusion is widely used in clinical practice to treat patients with massive blood loss. In 2014 alone, about 1 million liters of donor plasma were procured in Russia [1]. Plasma for transfusion is quarantined for 6 months at a temperature of -40°C. Despite a complex multi-stage analysis of plasma before and after the quarantine period, clinicians know that complications after a plasma transfusion still may develop. The studies give examples of such complications after plasma transfusion as poisoning of unknown etiology, nonhemolytic reactions, sepsis, transfusion-related acute lung injury [3]. Since causes of complications after plasma transfusion are often unclear, the search for additional parameters to assess the suitability of plasma for transfusion is still urgent
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