Abstract
Reversed-phase high-performance liquid chromatographic methods with UV-photodiode array or multiwavelength detection were developed for the analysis of secondary fungal metabolites mevinolin (also known as monacolin K, lovastatin or Mevacor) and fumagillin in fermentation broths. Samples obtained from screening experiments of Aspergillus fumigatus and Aspergillus terreus strains were analyzed for fumagillin and mevinolin, respectively. Isocratic and gradient elution was used with acetonitrile-aqueous phosphoric acid or acetonitrile-phosphate buffer eluents. Sample preparation was accomplished by extraction of the whole fermentation broth with an acid-organic solvent mixture. Similarly, bacterial degradation products of 2,4,6-trichlorophenol obtained from microbial remediation processes were analyzed by reversed-phase HPLC with UV-photodiode array detection. Extraction of the analyte from solid samples was done by solvent extraction and solid-phase extraction clean-up using octadecyl-modified silica sample preparation cartridges.
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