MONITORING OF SALT TOLERANCE RESPONSES OF DIFFERENT MAIZE INBRED LINES USING PROTEIN PROFILE AND PROTEIN SPECTROFLUORESCENCE

Abstract

Seedlings of Six maize inbred lines CML 511, CML 448, CML 444, CML 395, CML 254 and CML 216 were used to analyze salt stress of (150 mM NaCl). The analyses of RAPD results showed 84.6% of polymorphic fragments. Accessions of CML 511 and CML 448 were included in one main cluster with genetic similarity of 0.65. The genetic similarity between CML 444 and CML 395 was 0.72, the other accessions of CML 254 and CML 216 had genetic similarities of 0.67 and 0.62, respectively. Leaf proteins were studied by one-dimensional SDS-PAGE. Under non stress conditions, the minor band of 45 kDa was not expressed in varieties of CML 254 and CML 216 while band of 23 kDa were expressed in all accessions except of CML 216 accession. Under stress conditions high molecular weight bands were disappeared and the major protein bands were located at the medium molecular weight range. Protein spectrofluorescence of leaf protein samples show main emission peak at about 320 nm in both conditions of salt stress and control. However, the emission intensities showed some variations among different maize lines in alternative conditions of control and salt stress. This might be referred to change in protein interactions. Inbred lines of CML 511 and CML 448 exhibit closely related fluorescence pattern, this is in context of their genetic similarities as has deduced by RAPD results.