Abstract
In this work, p-hydroxybenzoic acid hydrazide has been used as a reagent to determine reducing sugars using flow-injection volume, flows and reagent concentration were examined for their optimum conditions. Systems catalysed by bismuth for low analyte concentrations and non-catalysed for higher levels, containing only a single sugar (sampling frequency 60−80 h −1) and a system with a longer residence time (sampling frequency 30 h −1) in which the different responses of various sugars can be minimised, are described. Samples (fruits and beverages) measured with the latter system in the calibration range 0.08−0.6 g/1 gave a typical R.S.D. of 2%.
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