Monitoring of indicator and multidrug resistant bacteria in agricultural soils under different irrigation patterns

Abstract

The presence of multidrug-resistant bacteria has been proposed as an environmental impact indicator of wastewater use in agricultural soils. Nevertheless, the effect of untreated wastewater discharge on occurrence and persistence of these type of bacteria in soils is unclear. In this study, multidrug-resistant bacteria and microbial indicators of fecal contamination (coliforms and Salmonella) were determined at different depths (0–15, 15–30 and 30–50cm), in active agricultural soils under different untreated wastewater irrigation pattern (a zone with past and present wastewater irrigation; a zone with wastewater irrigation until 2003; and a zone that had never been irrigated with wastewater) in Chihuahua, Mexico. A total of 94 multidrug-resistant strains were recovered from all tested soil samples, even from those that have never been irrigated with wastewater. Higher presence (P<0.05) of multidrug-resistant bacteria was found in soils currently irrigated with untreated wastewater than in soils that has not been irrigated with wastewater for more than 10 years; the later had presence of multidrug-resistant bacteria that was statistically higher than soils that had never been irrigated with wastewater. Wastewater irrigation showed an impact on proliferation of antibiotic-resistant Gram negative bacteria in soils actually irrigated but not in Gram positive bacteria. No statistical difference was found for coliforms and Salmonella between the three soil groups. The effect of depth in multidrug-resistance bacteria presence was negligible. Although multidrug-resistant bacteria was also isolate from soils without wastewater irrigation, their persistence in a highest number in soils irrigated with wastewater suggest that use of untreated wastewater for irrigation enhance the proliferation of multidrug-resistant microorganisms which can be monitoring in soils even after a long period of time after the last irrigation.