Monitoring of in vitro interaction studies of enalapril with hypoglycemic agents by LC-UV

Abstract

Monitoring of in vitro interaction studies of enalapril with hypoglycemic agents by LC-UV Najma Sultana,1 Safila Naveed,1,2 M Saeed Arayne31Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan; 2Faculty of Pharmacy, Jinnah University for Women, Karachi, Pakistan; 3Department of Chemistry, University of Karachi, Karachi, PakistanAbstract: The coadministration of antihypertensive and antidiabetic drugs is common, as both of these ailments are synergistic to each other and often occur together. In the present paper, we describe in vitro drug interactions of enalapril, an antihypertensive drug, with the hypoglycemic agents, metformin, glibenclamide, and glimepiride. These studies were carried out using an isocratic reversed phase high-performance liquid chromatographic method using a C18 column with ultraviolet detection at 230 nm. The system was operated at room temperature using a mobile phase consisting of methanol:water (70:30) adjusted to pH 2.5 with o-phosphoric acid with a flow rate of 1 mL minute-1. The assay was reproducible, linear (concentration range of 2.5–100 µg mL-1) with a correlation coefficient of 0.9999 and an accuracy rate of 98%–102%. The results from the reversed phase high-performance liquid chromatographic method clearly indicated that the availability of enalapril was unaffected by the simultaneous administration of hypoglycemic agents. Hence, the two drugs can be safely administered with one another.Keywords: enalapril, antihypertensive agent, hypoglycemic agents, drug interactions, RP-HPLC