Abstract
Deer (Cervidaei) belong to the most important species used as farmed animals. We focused on assesing the genetic diversity among five deer populations. Analysis has been performed on a total of 183 animals originating from Czech Republic, Hungary, New Zealand, Poland and Slovak Republic. Genetic variability were investigated using 8 microsatellite markers used in deer. Statistical data of all populations we obtained on the basis of Nei statistics, using by POWERMARKER 3.23 programme. Graphical view of relationships among populations and individuals in the populations was obtained using the Dendroscope software. Molecular genetic data combinated with evaluation in statistical programmes could lead to a complex view of populations and diffrences among them.
Highlights
Deer (Cervidaei) belong to the most important species used as farmed animals
We focused on assesing the genetic diversity among five deer populations
In Magyar allatorvosok lapja, vol 129, 2007, no. 11, p. 656-660, ISSN 0025-004X
Summary
DNA bola izolovaná z chlpových cibuliek z celkového počtu 183 farmových jeleňov pochádzajúcich z Českej Republiky (50), Maďarska (30), Nového Zélandu (45), Poľska (45) a Slovenskej Republiky (13). V modifikovanom PCR multiplexe podľa Ernsta (2008) bolo použitých 8 mikrosatelitných markerov. PCR reakcia prebiehala na prístroji PTC-150 MinicyclerTM v krokoch: predinkubácia pri 95°C, 5 min.; 30 cyklov pozostávajúcich z denaturácie (95°C, 30 s.), aneling (59°C, 90 s.) a extenzie (72°C, 90 s.), záverečná extenzia pri 72°C, 1 min. Fragmentyčná analýza PCR produktov prebiehala pomocou prístroja ABI. Genetická diverzita populácií bola vyhodnotená pomocou Nei (1983) štatistiky v programe POWERMARKER 3.23 (Liu and Muse, 2005) - počet alel (NA), počet genotypov, pozorovaná (Ho) a očakávaná heterozygozita (He) a Hardy-Weibergová rovnováha (HWE). Dendrogramy boli zostrojené pomocou softvéru Dendroscope (Huson et al, 2007). Tabuľka 1 Zoznam primerov , farbička používaná pri fragmentačnej analýze, sekvencia primerov
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