Abstract
Abstract Adeniran, G.A. and Oyejide, A. 1995. Monitoring of antibody response to Marek's disease vaccination in chickens by indirect ELISA. J. Appl. Anim. Res., 8: 55–62. An indirect Marek's disease ELISA “MD-ELISA” was standardised for the detection of antibodies in chicken. The ELISA proved to be specific since it discriminated significantly between sera from MD and Newcastle Disease (NCD)-vaccinated chickens. When applied to the seromonitoring of cockerel flocks, routinely immunised with MD or NCD vaccines, the MD-ELISA detected high maternal antibodies to MD in all birds at one week of age. Subsequently, the MD-vaccinates demonstrated a progressive rise in active antibody response which peaked at 5 weeks. However, such MDV antibodies had waned significantly in NCD vaccinates by week 3. It is concluded that the new MD-ELISA has potential for use as a screening assay for the presence of antibodies to Marek's Disease in chicken flocks.
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