Abstract
To monitor dopamine neural activities in the ventral tegmental area (VTA) in response to nicotine, we designed a dopamine platform based on a microimaging device and a microdialysis system. We measured dopamine (DA) release in the prefrontal cortex (PFC) and the nucleus accumbens (NAc) by a micro dialysis system and monitored DA neurons activities by fluorescence imaging in the VTA using a microimaging device simultaneously. GCaMP6 transgenic mice were used in this study, and the change in the fluorescence ratio intensity associated with nicotine administration was estimated. Our results suggested that nicotine administration increased the DA neurons activity in the VTA as well as the DA release in the PFC and the NAc. The proposed platform has the potential to monitor neural activities in the deep brain regions of rodents.
Highlights
Dopamine (DA) is a neurotransmitter which plays critical roles in pleasure, motivation, and learning
GCaMP6 IMAGING OF DA NEURON AND MEASUREMENT OF DA RELEASE WITH NICOTINE INTAKE The relationship between DA neuron activity in the ventral tegmental area (VTA) and DA release in the prefrontal cortex (PFC) or the nucleus accumbens (NAc) has been investigated by several groups using several methods including electrophysiology, imaging, and microdialysis [1], [2], [5], [22]
We focused on the interaction of the fluorescence difference ( F) and the amount of DA release (% Baseline) to the amount of nicotine intake
Summary
Dopamine (DA) is a neurotransmitter which plays critical roles in pleasure, motivation, and learning. DA neurons in the VTA have a strong connection to the NAc and the PFC for DA release (Fig. 1). This connection has been well established, quantifying the simultaneous dynamics of the DA neural network and activities within multiple regions, including regions in deep brain such as the VTA and the NAc, has been challenging. To overcome this challenge, several measurement systems have been developed including the combination of a microdialysis system and positron emission tomography (PET) technology [4]. DA release in the PFC was quantified using a microdialysis probe while brain activities by PET were monitored
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