Monitoring glycosylation of therapeutic glycoproteins for consistency using highly fluorescent anthranilic acid.

Abstract

AbstractUnderstanding the structure and function of proteins is a major area of interest and significance in biochemistry. Many proteins frequently reported in scientific journals are glycosylated. The oligosaccharide chains attached to the proteins may elicit important functional or structural properties (1,2). The characterization of oligosaccharide chains in addition to its protein part is important in order to fully understand the structure and function of glycoproteins. There are cases where either no apparent function is obvious or not yet identified for the carbohydrate part. However, characterization of carbohydrate units is necessary to define the therapeutic glycoprotein structure for regulatory purposes. This may include studying the extent of glycosylation in terms of its monosaccharide composition (including sialic acids and percent carbohydrate) and the nature of oligosaccharide chains.KeywordsSialic AcidAnthranilic AcidMonosaccharide CompositionOligosaccharide ChainSodium CyanoborohydrideThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.