Abstract
ABSTRACT Sugarcane (Saccharum officinarum L.) plantlets derived through direct somatic embryogenesis were evaluated using random amplified polymorphic DNA (RAPD) markers to assess genetic fidelity. Thirty-five arbitrary 10-mer oligonucleotide primers produced amplification products and seventeen were used to screen for variation among 100 regenerants. In general, 4384 bands were scored of which only 125 bands were polymorphic. With all the primers used, extent of polymorphism ranged from 0.86 to 8.45%. All the RAPD profiles were monomorphic and similar with very low variation among the plantlets. The results suggest that direct somatic embryogenesis as a method can generate plants with maximum uniformity and that RAPD can be useful as a tool to check genetic variation among sugarcane regenerants.
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