Abstract
NAD + is degraded by horse liver alcohol dehydrogenase in a reaction with p-methylbenzyl hydroperoxide, which acts as a pseudo-substrate. An unstable intermediate product (called compound I) is formed, and this is converted into the final product NADX. The reaction can be monitored by capillary electrophoresis. NADX migrates as two well resolved peaks (NADX 1 and NADX 2), which belong probably to the tautomers of the same substance. The intermediate product, compound I, has the same effective mobility as the conformer NADX 2 and it was impossible to separate compound I from NADX 2. The spectra taken during the separation show clearly that the “9-min” peak contains different compounds during the course of the reaction and the NADX 2 is not identical with compound I.
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