Monitoring Antifungal Agents of Artemisia annua against Fusarium oxysporum and Fusarium solani, Associated with Panax notoginseng Root-Rot Disease.

Yu-Nan Ma,Qing-Qing Li,Fu-Rong Xu,Yong-Xian Cheng,Chuan-Jiao Chen,Xian Dong

doi:10.3390/molecules24010213

Abstract

Root rot of Panax notoginseng has received great attention due to its threat on the plantation and sustainable utilization of P. notoginseng. To suppress the root-rot disease, natural ingredients are of great importance because of their environment friendly properties. In this study, we found that the methanol extract from Artemisia annua leaves has strong antifungal effects on the growth of Fusarium oxysporum and Fusarium solani resulting into root-rot disease. Essential oil (EO) thereof was found to be the most active. GC-MS analysis revealed 58 ingredients and camphor, camphene, β-caryophyllene, and germacrene D were identified as the major ingredients. Further antifungal assays showed that the main compounds exhibit various degrees of inhibition against all the fungi tested. In addition, synergistic effects between A. annua EO and chemical fungicides were examined. Finally, in vivo experiments were conducted and disclosed that P. notoginseng root rot could be largely inhibited by the petroleum ether extract from A. annua, indicating that A. annua could be a good source for controlling P. notoginseng root-rot.

Highlights

Panax notoginseng is a perennial herb (Araliaceae)
To satisfy the requirement of in vivo experiment, the petroleum ether extract from A. annua leaves, which simulates the components of A. annua Essential oil (EO), was prepared and mixed into the culture medium
The results reveal that A. annua EO is the most active, whereas the inhibitory effects of water soluble substances are negligible (Figure 3)

Summary

Introduction

Panax notoginseng is a perennial herb (Araliaceae). Its dry roots and rhizomes are used as medicinal parts known as San-Qi in Chinese. CROs could be resulted from multiple factors, of which, the root-rot disease caused by a single pathogen or a combination of pathogens could not be ignored. It is the most destructive and could result in yield reduction, no harvest, and a loss of active ingredients [4]. The pathogen fungi in the P. notoginseng rhizosphere have been investigated, indicating that F. solani, F. oxysporum, and C. destructans are the main pathogens of P. notoginseng root-rot disease [5,6,7].

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