Abstract

A new device based on the Raspberry-Pi to monitor the locomotion of Arctic marine invertebrates and to analyze chronobiologic data has been made, tested and deployed. The device uses infrared sensors to monitor and record the locomotor activity of the animals, which is later analyzed. The software package consists of two separate scripts: the first designed to manage the acquisition and the evolution of the experiment, the second designed to generate actograms and perform various analyses to detect periodicity in the data (e.g., Fourier power spectra, chi-squared periodograms, and Lomb–Scargle periodograms). The data acquisition hardware and the software has been previously tested during an Arctic mission with an arctic marine invertebrate.

Highlights

  • The spontaneous locomotor activity of animals is a useful parameter in ecological studies [1] and in chronobiology, in particular for circadian analysis

  • We present a new system for the acquisition and analysis of the behavioral data in chronobiological studies, based on an inexpensive hardware, coupled to a Raspberry-Pi computer

  • The Raspberry Pi has a limited amount of computing resources and the acquisition of several signals has a high computational cost

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Summary

Introduction

The spontaneous locomotor activity of animals is a useful parameter in ecological studies [1] and in chronobiology, in particular for circadian analysis. We present a new system for the acquisition and analysis of the behavioral data in chronobiological studies, based on an inexpensive hardware, coupled to a Raspberry-Pi computer. In this Pi article, we present a new system the acquisition and analysis provides of the behavioral data in This configuration an inexpensive, chronobiological studies, based on an inexpensive a Raspberry-Pi computer platform-independent, and open-source platform tohardware, circadian coupled analysis,tosimilar to those utilized in. The design aim was to integrate algorithms user specification of the statistical stringency in the signal detection This approach increases flexibility typically used forcapability the analysis of periodic in the analytical of the software.signals with both specific functions for signal filtering, and functions allowing the conditions statistical stringency in theIslands signal using detection. This device does not filter or oxygenate the water, and relies on regular water changes by the user

Aquaria
Electronics
Connector
Software
Software Package
Main Software
Real Time Analysis
A Posteriori Analysis
Comparison with Previous System
Classical
10. Temperature
12. Current
Experiment,Results
Animals
Experimental Procedure
Discussion
Conclusions
Methods
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