Abstract
Objective To monitor the drug resistance status of yersinia isolates from fresh meat, grasp the drug resistance spectrum and trend of Yersinia isolates in Our province, and provide important reference data for guiding the rational application of antibiotics in human clinical practice and controlling the spread of drug resistant strains. Methods A total of 600 pieces of fresh pork, beef and chicken were collected from several farmers' markets and supermarkets in Changchun every month for 15 months from August 18th to October 19th. 80 strains of Yersinia strains were detected, and MIC concentrations of drug resistance were detected by microbroth dilution with 15 antibiotic sensitive plates. Results 80 strains of Yersinia strains were counted. The total drug resistance rate of Yersinia pestis was 98.8%, and cefazolin (CFZ) had the highest drug resistance rate of 92.5.% (74/80). Erythromycin (ERY) was followed by 58.8% (47/80). Ampicillin (AMP) 57.5% (46/80); Naproxil acid (NAL) and cefoxitin (CFX) were 28.8 (23/80) and were somewhat resistant to other antibiotics. But all strains were sensitive to imipenem (IMP) and gentamicin (GEN). There were 28 strains with multiple drug resistance, accounting for 35.4% (28/79). Conclusion There are multiple antibiotic resistance in Yersinia strains in this monitoring, and the multiple drug resistance is serious and the resistance spectrum is diverse. In order to ensure food safety and human health, great attention should be paid to and enhanced drug resistance monitoring of Yersinia.
Highlights
monitor the drug resistance status of yersinia isolates from fresh meat
grasp the drug resistance spectrum and trend of Yersinia isolates in Our province
beef and chicken were collected from several farmers' markets and supermarkets in Changchun every month
Summary
摘要:目的 对来源于鲜肉食品中耶尔森菌分离株的耐药状况进行监测,掌握我省耶尔森菌的耐药谱和耐药趋势,为指 导抗生素在人类临床中的合理应用,控制耐药菌株的传播提供重要的参考性数据。方法 从18年8月到19年10月历经15 个月,每个月采集长春市多家农贸市场和超市销售的鲜猪肉、牛肉和鸡肉共600份。从中检测到的耶尔森菌菌株80株, 利用微量肉汤稀释法,用含有15种抗生素的药敏板进行了抗药性最低抑菌浓度(MIC)检测。结果 对80株耶尔森菌抗 药性进行统计,检出耶尔森菌总耐药率为98.8%,其中耐药率最高的为头孢唑林(CFZ)92.5.%(74/80);其次是红 霉素(ERY)58.8%(47/80);氨苄西林(AMP)57.5%(46/80);萘啶酸(NAL)和头孢西丁(CFX)为28.8(23/80), 对其他抗生素有一定程度的耐药。但所有菌株对亚胺培南和庆大霉素全部敏感。多重耐药有28株,占(28/79)35.4%。 氏菌、小肠结肠炎耶氏菌与假结核耶氏菌等十余个菌种, 这是一类革兰阴性小杆菌。其中鼠疫耶氏菌、小肠结肠炎 耶氏菌与假结核耶氏菌对人类的致病性已明确[1,2,3]。耶尔 森氏菌为动物肠道寄生菌,广泛存在于动物体内。它是目 前国际社会广泛关注的一种食源性病原菌。该菌广泛分布 [4,5,6]。目前在我国食品中耶尔森菌的检出率相对较低,系 上可疑菌落经分解尿素的菌株阳性培养物,使用API20E 统的监测数据不多。为了解耶尔森菌菌株在食品中的污染 生化试剂条鉴定进行系统生化鉴定。所有耶尔森菌属的菌 MIC板[7,8,9]。将质控菌株和备检菌株分别接种于哥伦比亚 血琼脂平板,37 °C 24 h 纯培养。挑取 3~5 个纯新菌
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