Abstract
The yeast strain Moniliella spathulata SBUG-Y 2180 was isolated from oil-contaminated soil at the Tengiz oil field in the Atyrau region of Kazakhstan on the basis of its unique ability to use crude oil and its components as the sole carbon and energy source. This yeast used a large number of hydrocarbons as substrates (more than 150), including n-alkanes with chain lengths ranging from C10 to C32, monomethyl- and monoethyl-substituted alkanes (C9–C23), and n-alkylcyclo alkanes with alkyl chain lengths from 3 to 24 carbon atoms as well as substituted monoaromatic and diaromatic hydrocarbons. Metabolism of this huge range of hydrocarbon substrates produced a very large number of aliphatic, alicyclic, and aromatic acids. Fifty-one of these were identified by GC/MS analyses. This is the first report of the degradation and formation of such a large number of compounds by a yeast. Inoculation of barley seeds with M. spathulata SBUG-Y 2180 had a positive effect on shoot and root development of plants grown in oil-contaminated sand, pointing toward potential applications of the yeast in bioremediation of polluted soils.Key points• Moniliella spathulata an oil-degrading yeast• Increase of the growth of barley
Highlights
The yeast SBUG-Y 2180 was isolated from a sample of oilcontaminated soil from the Tengiz oil field in the Atyrau region of Kazakhstan
The yeast SBUG-Y 2180 was enriched from a contaminated soil sample of the Tengiz oil field in the Atyrau region of Kazakhstan on Tengiz crude oil–containing media according to the method of Joo et al (2008), and isolated by plating 0.1 mL of the enriched cultures on Sabouraud agar (Merck, Germany)
The ITS sequence was deposited in GenBank: Moniliella spathulata SBUG-Y 2180 (B1) Accession No MT003285
Summary
The yeast SBUG-Y 2180 was isolated from a sample of oilcontaminated soil from the Tengiz oil field in the Atyrau region of Kazakhstan. The oil contains a large proportion of gas, and is rich in H2S. These properties of the Tengiz reservoir are serious challenges for its exploitation, even when the latest oil production technology is used. The most dangerous accident was an explosion in 1985, which produced a 200-m-high fire column that burned for more than a year (Pala 2001). This and other accidents have led to the pollution of the surrounding soil
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