Abstract
Diabetes mellitus increases oxidative stress and formation of advanced glycation endproducts (AGEs) both of which underlie vascular complications by interfering with angiogenesis required for wound-healing. Momordica charantia (MC) prevents AGE formation and promotes diabetic wound-healing in animals. This study investigates the effects of MC pulp (MCP), flesh (MCF) and charantin using an in vitro model of angiogenesis. Cultured bovine aortic endothelial cells were exposed to bovine serum albumin (BSA)-derived AGEs. MC extracts increased cell proliferation, migration (using wound-healing assay) and tube formation (using Matrigel™-embedded 3D culture) in a dose-dependent manner, and these effects were associated with increases p-ERK1/2 signaling. These effects were inhibited by MCP, MCF and charantin extracts. Blocking the receptor for AGEs (RAGE) inhibited the MC extract-induced ERK1/2 phosphorylation and tube formation, indicating the crucial role of RAGE in MC pro-angiogenic effects. In conclusion, MC extracts and charantin exert direct pro-angiogenic effects mediated through RAGE with inhibitory activity on anti-angiogenic effects of BSA-AGEs. Further studies are needed to understand the therapeutic potential of MC extracts in diabetic wound-healing.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
