Molt-inhibiting hormone (MIH) gene from the green mud crabScylla paramamosainand its expression during the molting and ovarian cycle

Abstract

Mud crab is a group of commercially important species that are found throughout the Indo-Pacific region. In this study, the full-length cDNA of molt-inhibiting hormone (Scp-MIH) was cloned from the green mud crab, Scylla paramamosain. The conceptually translated peptide precursor consists of a 78-residue mature peptide, preceded by a 35-residue signal peptide. The mature peptide shares a high identity with the other known MIHs, and contains six conserved cysteine residues that have been proposed to be functionally critical for MIH activity. Analysis of genomic sequence revealed that Scp-MIH gene was organized in a 3 exons/2 introns manner. Quantitative real-time PCR showed that Scp-MIH transcripts were detected mainly in the eyestalk ganglion and brain. During the molting cycle, Scp-MIH mRNA increased significantly from postmolt stage to intermolt stage, then decreased sharply at premolt stage. It implies that MIH is closely related to ecdysteroid production in S. paramamosain. In the ovarian cycle, Scp-MIH transcripts in some neural tissues (eyestalk ganglion and brain) increased from stage I to stage II, and reached the peak value at stage III, then declined at later stage. It suggests that MIH might be involved in the ovarian maturation in S. paramamosain.