Abstract
Moloney cell surface antigen (MCSA) is serologically detectable on Moloney murine leukemia virus (Mo-MuLV)-induced lymphomas. It is probably related to the tumor-associated transplantation antigen of these lymphomas. We characterized MCSA by using the typing antiserum in radioimmunoprecipitations followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Surface-iodinated Mo-MuLV-infected cells were used to identify the molecular nature of MCSA. It appeared that MCSA was expressed as an entity of 82,000 apparent molecular weight (p82 MCSA). Translation of size-fractionated mRNAs from infected cells in oocytes of Xenopus laevis showed that p82 MCSA was made on an mRNA of the same size (22 S) as that coding for the Mo-MuLV env proteins. Moreover, p82 MCSA was processed in oocytes to proteins exactly comigrating in the gel with gp70, p15E, and p12E. Competition experiments with unlabeled virus showed that MCSA and the MCSA-related env-like proteins made in oocytes were not related to any of the Mo-MuLV structural proteins. We concluded that MCSA has the properties of the product of the env gene of a murine C-type virus different from Mo-MuLV.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
