Molekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbiji

Dragana Milošević,Zorica Nikolić,Ivana Stanković,Branka Krstić,Maja Ignjatov,Ana Marjanović-Jeromela,Gordana Tamindžić

doi:10.5937/ratpov56-23514

Abstract

In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank.

Highlights

Considering that Turnip yellow virus (TuYV) is one of the most important and widespread viruses in brassica crops worldwide, the virus presence in oilseed rape crop in Serbia (Milošević et al, 2015), as well as the common presence of a lot of aphids known as virus vectors, TuYV potentially represents a limiting factor for a successful production of mustard plants in Serbia
Sample collection In 2018, mustard leaf samples showing virus-like symptoms, including mild reddening of leaf margins followed by mild yellowing leaves, were collected from two crops at two different localities: Rimski Šančevi (South Bačka District) and Senta (North Banat District) in Serbia
This study reports the natural occurrence of TuYV on B. nigra and S. alba in Serbia for the first time

Summary

Introduction

Considering that TuYV is one of the most important and widespread viruses in brassica crops worldwide, the virus presence in oilseed rape crop in Serbia (Milošević et al, 2015), as well as the common presence of a lot of aphids known as virus vectors, TuYV potentially represents a limiting factor for a successful production of mustard plants in Serbia. Sample collection In 2018, mustard leaf samples showing virus-like symptoms, including mild reddening of leaf margins followed by mild yellowing leaves, were collected from two crops at two different localities: Rimski Šančevi (South Bačka District) and Senta (North Banat District) in Serbia.

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