Molecularly imprinted solid phase extraction using stable isotope labeled compounds as template and liquid chromatography–mass spectrometry for trace analysis of bisphenol A in water sample

Abstract

We have developed a molecularly imprinted polymer (MIP) using a stable isotope labeled compound as the template molecule and called it the “isotope molecularly imprinted polymer” (IMIP). In this study, bisphenol A (BPA) was used as the model compound. None imprinted polymer (NIP), MIP, dummy molecularly imprinted polymer (DMIP) and IMIP were prepared by the suspension polymerization method using without template, BPA, 4- tert-butylphenol (BP) and bisphenol A-d 16 (BPA-d 16), respectively. The polymers were subjected to molecularly imprinted solid phase extraction (MI-SPE), and the extracted samples were subjected to liquid chromatography–mass spectrometry (LC–MS). Although the leakage of BPA-d 16 from the IMIP was observed and that of BPA was not observed. The selectivity factors of MIP and IMIP for BPA were 4.45 and 4.43, respectively. Therefore, IMIP had the same molecular recognition ability as MIP. When MI-SPE with IMIP was used and followed by LC–MS in the analysis of river water sample, the detection limit of BPA was 1 ppt with high sensitivity. Moreover, the average recovery was higher than 99.8% (R.S.D.: 3.7%) by using bisphenol A- 13C 12 (BPA- 13C 12) as the surrogate standard. In addition, the IMIP were employed in MI-SPE of BPA in river water sample by LC–MS. The concentration of BPA in the river water sample was determined to be 32 pg ml −1. We confirmed that it was possible to measure trace amounts of a target analyte by MI-SPE using IMIP.