Abstract
A new and selective sorbent for molecularly imprinted polymer solid-phase extraction (MIP-SPE) was prepared to extract meropenem from plasma and urine samples. The extracted analyte was analyzed by high-performance liquid chromatography (HPLC) coupled with photodiode array detection. Methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linker, azobisisobutyronitrile (AIBN) as an initiator and meropenem as a template molecule were used for the MIP preparation. Imprinted meropenem molecule was removed from the polymeric structure using acetonitrile in water (1:9, v/v), as the eluent solvent. Under the optimized conditions, the limit of detection (LOD) and limit of quantification (LOQ) were 35 and 120μg L-1, respectively. The developed MIP-SPE method demonstrates that it could be applied for the determination of meropenem in plasma and urine samples. Keywords: Molecularly imprinted polymer, solid-phase extraction, meropenem, human urine and plasma, high-performance liquid chromatography.
Highlights
Meropenem is a broad-spectrum carbapenem antibiotic.[1]
As announced in the scientific notice,[5] the MIC for the meropenem is very different from one bacteria to another, but very often it is effective in the range 0.5–4 μg mL–1 against most sensitive germs
Selectivity Test Ceftazidime, cefixime and isoniazid were selected as competitive compounds to estimate the selectivity of Molecularly imprinted polymers (MIPs) for meropenem
Summary
Meropenem is a broad-spectrum carbapenem antibiotic.[1]. It is active against Gram-positive and Gram-negative bacteria.[2]. Meropenem has advantages in the intensive care unit (ICU), notably in critically ill patients with renal failure, a population at risk for accumulating co-administered drugs or metabolites.[8]
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