Abstract
Lactose present in milk is metabolized by many microorganisms and converted into lactic acid. Lactic acid concentration is related to the total bacterial count and can be a useful indicator of milk quality and state of preservation. Thus, lactate-imprinted polymer prepared by cross-linking of polyamine hydrochloride with epichlorohydrin in the presence of lithium lactate was synthesized. The prepared polymer achieved 7.7 fold selectivity over the non-imprinted polymer in water. Imprinted polymer prepared against lithium lactate retained selectivity towards both stereo forms of lactic acid. The prepared polymer is stable up to 9 months at room temperature and can be used in chromatographic column for preconcentration of lactate and its estimation in formalin preserved milk samples.
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