Molecularly Imprinted Polymer Beads: Suspension Polymerization Using a Liquid Perfluorocarbon as the Dispersing Phase

Abstract

A suspension polymerization technique suitable for molecular imprinting is described, based on the use of a liquid perfluorocarbon as the dispersing phase. This dispersant does not interfere with the interactions between functional monomers and print molecules required for the recognition process during molecular imprinting. The method produces polymer beads, with almost quantitative yield, which can be used after only a simple washing step. An acrylate polymer with perfluorocarbon and poly(oxyethylene) ester groups was used to stabilize an emulsion of functional monomer, cross-linker, print molecule, initiator, and porogenic solvent in perfluoro(methylcyclohexane). Initiation of polymerization by UV irradiation resulted in polymer beads. The average bead size could be controlled between about 50 and 5 μm by varying the amount of stabilizing polymer. SEM of the beads indicated spherical particles with morphology typical of beads made by suspension polymerization. The technique was applicable to a range of conditions typically used for molecular imprinting. A detailed chromatographic study of the polymer beads confirmed that α values and resolution factors were similar to those achieved with traditional ground and sieved imprinted polymers. Small (5 μm) beaded packings gave low back pressure and rapid diffusion, giving good separation even at high flow rates.