Abstract
2,4,6-trichlorophenol molecularly imprinted suspension polymer has been prepared and applied to the molecularly imprinted micro-solid-phase extraction procedure for selective preconcentration of phenolic compounds from environmental water samples. The influence of functional monomer, cross-linker, polymerization condition, porogen, and the ratio of template molecule and functional monomer to cross-linker on the size of the obtained particles were investigated. It was found that methyacrylic acid as functional monomer, divinylbenzene as cross-linker, the molar ratio of template molecule and functional monomer to cross-linker was 1:4:20, the amount of AIBN was 100 mg, ultraviolet radiation at 365 nm were the optimal conditions, and at these conditions, the polymers had the best adsorption efficiency and had the monodispersity of 2 - 3 μm microsphere particles. The characteristics of the MIMSPE method were valid by high performance liquid chromatography. This MIMSPE-HPLC method has been successfully applied to the direct preconcentration and determination of phenolic compounds (phenol, 4-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, pentachlorophenol) in environmental water samples.
Highlights
Imprinted polymers (MIPs) have attracted considerable research interests due to their predetermined selectivity toward a given analyte or a group of structurally related species
It was found that methyacrylic acid as functional monomer, divinylbenzene as cross-linker, the molar ratio of template molecule and functional monomer to cross-linker was 1:4:20, the amount of AIBN was 100 mg, ultraviolet radiation at 365 nm were the optimal conditions, and at these conditions, the polymers had the best adsorption efficiency and had the monodispersity of 2 - 3 μm microsphere particles
The characteristics of the MIMSPE method were valid by high performance liquid chromatography
Summary
Imprinted polymers (MIPs) have attracted considerable research interests due to their predetermined selectivity toward a given analyte or a group of structurally related species. The procedure involves crushing, grinding and sieving these resultant monoliths to an appropriate particle size for the particular studies which will be undertaken [1] These irregular particles generally give less efficient column packing for chromatography and often prove troublesome in chromatography process, when MIPs are used as affinity-based chromatography media, HPLC stationary phases or solid-phase extraction (SPE) media, it is desirable to prepare the spherical and mono-dispersed beads. Beaded MIPs may be obtained via the use of grafting procedures where one distinguishes between “grafting to” and “grafting from” techniques [7], the latter referring to polymerization initiated from a preformed polymer or from the surface of a solid support These techniques suffer from the presence of water or highly polar organic solvents during the polymerization procedure, which frequently decreases specific interactions between functional monomers and template molecules in most commonly applied non-covalent imprinting
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