Abstract
Formononetin is an isoflavone compound found in many traditional Chinese medicines that has a wide range of pharmacological activities. It is critical to develop a sensitive, accurate, and rapid determination method for in-depth formononetin research. A molecularly imprinted electrochemical sensor was constructed in this study. After the flexible electrode (ITO-PET) was modified with nitrogen-doped graphene (NG), the molecularly imprinted polymer (MIP) was electropolymerized on the surface using o-phenylenediamine (OPD) as the functional monomer and formononetin as the template. Under optimized experimental conditions, the MIP sensor detected formononetin selectively in 0.1 M phosphate buffer solution (PBS) with the linear range of 3 ~ 120 μM, a detection limit as low as 1.14 μM (S/N = 3), and good anti-interference ability and reproducibility. The analytical performance of the proposed MIP/NG/ITO-PET was evaluated for the detection of formononetin in real samples such as methanol extract of Radix Astragali and mouse plasma with good accuracy and precision.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
