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Abstract
The article presents a molecular genetic study of genomes of field isolates of rabies virus isolated in the Vladimir, Moscow, Tver, Nizhny Novgorod and Ryazan regions, with the aim of carrying out phylogenetic analysis. We studied 20 samples of purified PCR products containing the rabies virus nucleoprotein. The samples were provided by the Vladimir veterinary service. Sequencing and phylogenetic analysis of the gene showed that 12 fragments of isolates under study were close to the Central phylogenetic group of the rabies virus; namely - 5 isolates from the Vladimir region, 2 from the Nizhny Novgorod region, 2 from the Moscow region, and 3 from the Tver region. Eight studied isolates from the Nizhny Novgorod and Ryazan regions were attributed to the Eurasian phylogenetic group.
Highlights
В статье представлено молекулярно-генетическое исследование геномов полевых изолятов вируса бешенства, выделенных на территории Владимирской, Московской, Тверской, Нижегородской и Рязанской областей, с целью проведения филогенетического анализа
The article presents a molecular genetic study of genomes of field isolates of rabies virus isolated in the Vladimir, Moscow, Tver, Nizhny Novgorod and Ryazan regions, with the aim of carrying out phylogenetic analysis
We studied 20 samples of purified PCR products containing the rabies virus nucleoprotein
Summary
Зайкова О.Н.1, 3, Гребенникова Т.В.1, 3, Гулюкин А.М.2, Шабейкин А.А.2, Полякова И.В.2, Метлин А.Е.4. Молекулярно-генетическая характеристика полевых изолятов вируса бешенства, выявленных на территории Владимирской, Московской, Тверской, Нижегородской и Рязанской областей. MOLECULAR–GENETIC CHARACTERIZATION OF FIELD ISOLATES OF RABIES VIRUS IDENTIFIED IN THE TERRITORY OF VLADIMIR, MOSCOW, TVER, NIZHNY NOVGOROD AND RYAZAN REGIONS. The article presents a molecular genetic study of genomes of field isolates of rabies virus isolated in the Vladimir, Moscow, Tver, Nizhny Novgorod and Ryazan regions, with the aim of carrying out phylogenetic analysis. Sequencing and phylogenetic analysis of the gene showed that 12 fragments of isolates under study were close to the Central phylogenetic group of the rabies virus; namely − 5 isolates from the Vladimir region, 2 from the Nizhny Novgorod region, 2 from the Moscow region, and 3 from the Tver region.
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