Abstract
Objective of the study is to investigate phenotypic and molecular-genetic features and perform whole genome sequencing of Y. pestis strains isolated in Vietnam. Materials and methods. Studied were phenotypic and genotypic peculiarities of 20 plague agent strains isolated in different prefectures of Vietnam. Carried out was SNP-analysis of the strains, sequenced were genomes of 8 Y. pestis strains. Results and conclusions. Based on the results of studies of differential biochemical characteristics all the investigated strains were attributed to oriental biovar of the main subspecies of plague agent, which was confirmed by the presence of marker indel mutation – deletion of 93 bps in glpD gene. Investigated was also the plasmid composition of the strains. On the basis of the conducted genome sequencing and SNP-analysis appurtenance of 19 out of 20 strains under examination was determined. They belong to 1.ORI2v phylogenetic branch, relative to the strains isolated in Yunnan Province, China, which points to their common origin. Identified was a marker SNP and developed the method of SNP-typing for 1.ORI2v strains from Vietnam.
Highlights
Цель работы состояла в изучении фенотипических, молекулярно-генетических особенностей и полногеномном секвенировании штаммов Y. pestis, выделенных во Вьетнаме
Objective of the study is to investigate phenotypic and molecular-genetic features and perform whole genome sequencing of Y. pestis strains isolated in Vietnam
Studied were phenotypic and genotypic peculiarities of 20 plague agent strains isolated in different prefectures of Vietnam
Summary
Л.М.Куклева, К.А.Никифоров, Ж.В.Альхова, Н.И.Романов, Н.Ю.Носов, А.В.Фадеева, А.Н.Малахаева, Н.А.Шарапова, З.Л.Девдариани, Ю.А.Попов, Г.А.Ерошенко. Молекулярно-генетические и фенотипические особенности штаммов возбудителя чумы, выделенных во Вьетнаме. Цель работы состояла в изучении фенотипических, молекулярно-генетических особенностей и полногеномном секвенировании штаммов Y. pestis, выделенных во Вьетнаме. Изолированные на территории Вьетнама, принадлежат к восточному биовару основного подвида Y. pestis, к филогенетической ветви 1.ORI, которая появилась более 200 лет назад. Что ветвь античного биовара 1.ANT во время второй пандемии чумы «Черная смерть» могла прокатиться по Европе до Азии и дать начало штаммам Y. pestis 1.ORI, вызвавшим текущую пандемию чумы [17]. Для оценки степени генетического родства штаммов возбудителя чумы, выделенных на территории Вьетнама в 1985–1987 гг., проведено изучение их генетического полиморфизма методом MLVA [8]. Цель настоящей работы состояла в изучении фенотипических, молекулярно-генетических особенностей и полногеномном секвенировани штаммов Y. pestis, выделенных во Вьетнаме
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
