Abstract

The molecular weights of two α-amylase isozymes [EC 3.2.1.1] (55°c α-amylases I and II) from B. stearothermophilus grown at 55°c and α-amylase [EC 3.2.1.1] (43°c α-amylase) from the same bacterium grown at 43°c were determined by different methods (gel filtration and sedimentation equilibrium). The α-amylase produced at 43°c contained both monomeric and dimeric forms of the enzyme. To elucidate the effects arising from dimerization, the enzymes were reduced and carboxymethylated and then the molecular weight was measured in 6.1M guanidine hydrochloride solution. The molecular weights of 43°c α-amylase and 55°c α-amylases I and II were determined by sedimentation equilibrim to be 44,000, 46,000, and 43,000 and by gel filtration (Sepharose 6B) to be 41,000, 44,000, and 41,000, respectively.

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