Abstract
A combined procedure, employing the Nazarian equation and the Griffith rapid equilibrium technique, is presented for the determination of molecular weights by low-speed sedimentation equilibrium using Rayleigh interference optics. This makes it possible to obtain a molecular weight measurement from one experiment in a relatively short period of time with high accuracy and comparatively simple calculations. Seven proteins ranging in molecular weight from 13,000 to 500,000 were examined, and the time to reach equilibrium was found to be linearly related to the log of the molecular weight, thus establishing the necessary criterion to use when deciding the duration of an equilibrium experiment in question. Finally, the Nazarian equation is shown to be ideally suited for use with the Edelstein-Schachman procedure for the determination of partial specific volumes.
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