Abstract
The family of T-cell immunoglobulin domain and mucin domain (TIM) proteins is identified to be expressed on T cells. A member of Tim family, Tim-3 (T cell immunoglobulin mucin 3) is selectively expressed on the surface of differentiated Th1 cells. Tim-3 might have an important role in the induction of autoimmune diseases by regulating macrophage activation and interacts with Tim-3 ligand to regulate Th1 responses. To determine the variation sites in the coding and promoter region of human Tim-3 gene, we performed variation scanning by direct sequencing using the genomic DNA isolated from the patients with asthma or allergic rhinitis and healthy controls without asthma and allergic rhinitis. We identified four single nucleotide polymorphisms (SNPs) including one novel SNPs (-1541C>T) and two variation sites (-1292_-1289delTAAA and -1282_-1278dupTAAAA) in the coding and promoter region of human Tim-3 gene in both the patients and healthy groups.
Highlights
Naive T helper cells differentiate into Th1 or Th2 cells by multiple factors, which include cytokine profile, type of antigen, delivery route of antigens, transcription factors and signaling pathways (O'Garra 2000; Ho and Glimcher 2002)
The results of above studies led us to identify the polymorphism of Tim-3 genes in a Korean
To determine the variation sites in the coding and promoter region of human Tim-3 gene, we performed variation scanning by direct sequencing using the genomic DNA isolated from 24 unrelated asthma patients, 26 unrelated allergic rhinitis patients and 24 unrelated healthy controls
Summary
Naive T helper cells differentiate into Th1 or Th2 cells by multiple factors, which include cytokine profile, type of antigen, delivery route of antigens, transcription factors and signaling pathways (O'Garra 2000; Ho and Glimcher 2002). All three of this gene family encodes cell surface glycoproteins with common structural motifs, including a signal peptide, Ig domain, mucin domain, transmembrane region and intracellular tail with phosphorylation sites. The results of above studies led us to identify the polymorphism of Tim-3 genes in a Korean.
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